CK20mRNA expression detected by FQ-PCR and its clinical significance in lymph nodes from pN0 colorectal cancer patients
- VernacularTitle:荧光定量PCR检测pNo期结直肠癌淋巴结中CK20mRNA的表达及其临床意义
- Author:
Weixiang CHEN
;
Shaohua FEI
;
Lei ZHU
;
Jian WU
;
Jun DING
;
Xiang TAO
- Publication Type:Journal Article
- Keywords:
Colorectal neoplasms;
Keratin;
Neoplasm metastases;
Lympha node;
Micrometastasis
- From:
Chinese Journal of General Surgery
2008;23(11):863-865
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study lymph node micrometastasis in N0 colorectal cancer patients and its clinical significance. Methods In this study, 548 lymph nodes obtained from 62 cases of No colorectal cancer undergoing curative operation were examined by fluorescent quantity polymerase chain reaction assay for the expression of cytokeratin 20 (CK20) mRNA. Results Micrometastasis was detected in 55 lymph nodes (10.0% ) of 24 cases (39%). According to lymph node anatomical locations, micrometastasis was identified in 15. 8%, 5.0% and 3.3% lymph nodes in group Ⅰ ,Ⅱ and Ⅲ (grouped by the distance from the tumor), respectively. Micrometastasis was correlated with invasion depth of primary tumor, but was notrelated to gender, age, tumor size, tumor site and differentiation. Conclusions The expression of CK20mRNA in lymph nodes in patients with No coloreetal cancer could be used to improve the accuracy of clinical staging, and provide information for rational adjuvant therapy.
