Study on the interaction between presenilin 1 and carboxyl terminus of Hsc70 interacting protein
- VernacularTitle:早老素1与热休克蛋白70同源蛋白羧基端相互作用蛋白
- Author:
Runzhong LIU
;
Xuelian YI
;
Shufang HUO
;
Yue YANG
;
Haibo HOU
;
Yunwu ZHANG
;
Shuigen HONG
;
Huaxi XU
- Publication Type:Journal Article
- Keywords:
Alzheimer disease;
Presenilin-1;
Heat-shock proteins
- From:
Chinese Journal of Geriatrics
2008;27(10):766-769
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo understand the pathological and physiological roles of Presenilin 1 (PS1) in Alzheimer's disease (AD) recurrence, and the interaction between PSI and carhoxyl terminus of Hsc70 interacting protein (CHIP). MethodsThe yeast two-hybrid system was applied to identify a novel PS1 interacting protein as CHIP. After pGBKT7-PS1-C203 bait plasmid and full fragement CHIP of pACT2-CHIP expression vector were constructed, the interaction between PSI and CHIP was tested by β-galactosidase assay, pGBKT7-PS1-C203 was co-transfected with pACT2-CHIP into 293T cells and the interaction between PS1 and CHIP was tested by co-immunoprecipitation and Western blot. ResultsSpecificity of the interaction between PS1 and CHIP was identified by β-galactosidase assay and co- immunoprecipitation. ConclusionsCHIP is able to modulate chaperone functions and the pathway of protein ubiquitination/degradation. CHIP may regulate a proper assembly of the γ-secretase complex through its interaction with PSI, which is helpful to elucidate the mechanism of AD pathology.
