A molecular biology study on the microorganism within teeth with failed root canal therapy
10.3969/j.issn.1001-3733.2010.01.023
- VernacularTitle:根管治疗失败病例根管内微生物的分子生物学检测
- Author:
Huibin SUN
;
Jing DENG
;
Yun WANG
;
Kun YANG
- Publication Type:Journal Article
- Keywords:
Failed root canal therapy;
Enterococcus faecalis;
Clinical feature;
Actinomyces israelii;
Prevotella nigrescens;
PCR test
- From:
Journal of Practical Stomatology
2010;26(1):96-99
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To examine the microbial flora within the root canals with failed root canal therapy by PCR test, to identify detection rate of bacteria and the predominant bacteria species, and to determine the association of the various species with clinical features. Methods: Forty roots with failed root canal therapy were selected for this study. According to clinical features the roots were divided into three groups: pain, fistula and symptomless. After removal of the root filling material, the microbial samples were taken from the canal and detected by PCR test. Results: The microbial flora within root canals of teeth with failed root canal therapy was found to be mixed, six kinds of bacteria were detected. Enterococcus faecalis was the most commonly recovered bacteria species. Significant associations were observed between Prevotella nigrescens and pain, while dependablity was observed between Actinomyces israelii and fistula. Conclusion: The main cause of root canal treatment failure is the persistent microorganisms. The composition of bacteria in teeth with failed root canal therapy has its own specificity.
