Effects of SDY-08 isolated from Dasyatis akajei on angiogenesis and tissue repair
- VernacularTitle:血管生成促进剂SDY-08的促组织修复作用
- Author:
Qiang WANG
;
Rundi MA
;
Lijian YU
;
Weiming SU
;
Laizhen HUANG
;
Xiaoyu ZHANG
;
Tingxi YU
- Publication Type:Journal Article
- Keywords:
Wound healing;
Physiologic neovascularization;
Vascular endothelial growth factor;
Dasyatis akajei;
SDY-08
- From:
Chinese Journal of Trauma
2008;24(11):900-904
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of SDY-08 isolated from Dasyatis akajei on tis-sue repair. Methods MTT assay was performed to measure the effect of SDY-08 on the growth of ECV-304 cells. The effect of SDY-08 on angiogenesis was detected in the chick embryochorioallantoic membrane (CAM). Mouse wound model was applied to investigate the effect of SDY-08 on tissue repair. Immunohistochemical staining assay and Western blotting were adopted to examine the expression changes of vascular endothelial growth factor (VEGF), Bcl-2 and Bax in wound tissues in response to SDY-08. Results The proliferation rates of SDY-08 at final concentration of 80 μg/ml promoting ECV-304 cells were 28.1%, 115.6% and 81.4% respectively at 12, 24 and 36 hours. The induction rate of angiogene-sis of CAM by SDY-08 at concentration of 1.6 mg/ml was 72.1%. SDY-08 at 0.5 mg/ml markedly in-duced acceleration of wound healing in mouse model two days in advance. SDY-08 up-regulated either ex-pressions of VEGF in trauma group or that of Bcl-2 and VEGF of ECV-304 cells, but down-regulated ex-pression of Box. Conclusions SDY-08 can significantly promote angiogenesis and tissue repair, which is closely correlated with its effect of up-regulating expressions of VEGF and Bcl-2 as well with that of down-regulating expression of Box.
