Effect of antisense oligodeoxynucleotide targeting survivin on hilar cholangiocarcinoma cell line FRH-0201
- VernacularTitle:Survivin反义寡核苷酸对肝门部胆管癌细胞株FRH-0201作用的研究
- Author:
Tao JING
;
Xuting ZHI
;
Xiaopeng WU
- Publication Type:Journal Article
- Keywords:
Bile duet neoplasms;
Survivinl Antisense oligodeoxynucleotide
- From:
Chinese Journal of Hepatobiliary Surgery
2008;14(9):633-635
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of antisense oligodeoxynueleotide targeting sur-vivin (survivin ASODN) on hilar cholangioearcinoma cell line FRH-0201 depressing the expression of survivin. Methods Survivin ASODN was transfected into hilar eholangiocarcinoma cell line FRH-0201by liposome. Morphologieal changes were observed by inverted phase contrast microscope. RT-PCR and Western blot methods were performed to detect the expressions of survivin mRNA and protein re-spectively. The changes in cell apoptosis were detected by flow cytometry. Results The expression of survivin mRNA and protein was significantly decreased in survivin ASODN group than that in the con-trol group(mRNA: 0.51-t-0. 03 vs 0. 82-t-0.02,P%0. 05～protein: 1.82-t-0.16 vs 3. 08--t-_0. 27, P-Q 0.05). The morphologieal apoptotic changes were observed and the apoptosis rate was increased (11.50+1.49% vs 0.39-+-0.08～, P%0.05). Conclusion Survivin AS()DN can induce hilar cholan-gioeareinoma cell line FRH-0201 into apoptosis by decreasing the expression of survivin.
