Study on expression and significance of hypoxia inducible factor-1α in liver tissues of rats with liver fibrosis
- VernacularTitle:缺氧诱导因子-1α在大鼠肝纤维化组织中的表达及其意义
- Author:
Yejin XU
;
Yongping CHEN
;
Li WAN
;
Mingqin LU
;
Ji LI
;
Xiaodong WANG
- Publication Type:Journal Article
- Keywords:
Transforming growth factor beta;
Vaccines;
Liver cirrhosis;
Hypoxia inducible factor-1;
Matrix metalloproteinase-9;
Immunohistochemistry;
Rats
- From:
Chinese Journal of Infectious Diseases
2008;26(11):651-655
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the expression and significance of hypoxia inducible factor (HIF)-1α,matrix metalloproteinase(MMP)-9 and transforming growth factor(TGF)-β1 in liver tissues of rats with hepatic fibrosis and the effects of TGF-β1 vaccine on the expressions of HIF-1α,MMP-9 and TGF-β1.Methods The hepatic fibrosis rat model was set up by dimethylnitrosamine (DMN).The rat model was injected with TGF-β1 vaccine.The expressions of HIF-1α,MMP-9 and TGF-β1 were detected by immunohistochemistry in rat fibrosis tissues after 6 weeks.Results The expressions of HIF-1α,MMP-9 and TGF-β1 in fibrosis model group and TGF-β1 vaccine group were all significantly higher than control group(P＜0.01),which was positively correlated with the fibrosis degree(r=0.911,0.814 and 0.836,respectively,P＜0.01).However,the expressions of HIF-1α,MMP-9 and TGF-β1 in TGF-β1 vaccine group were all significantly decreased than fibrosis model group (t=2.62,4.03,3.43;P＜0.01).Conclusion During the development of liver fibrosis,HIF-1α promotes the transcriptions and expressions of MMP-9 and TGF-β1.
