The Effect and Significance of Cinobufagin on the Expression of CD44s in Ovarian Carcinoma 3AO Cells
- VernacularTitle:华蟾素对卵巢癌3AO细胞CD44s表达的影响
- Author:
Shaoguang WANG
;
Xueqiang JIANG
- Publication Type:Journal Article
- Keywords:
Cinobufagin;
Ovarian carcinolna;
CD44s;
Invasive growth
- From:
International Journal of Traditional Chinese Medicine
2008;30(6):405-406
- CountryChina
- Language:Chinese
-
Abstract:
Objegtive To investigate the effect and significance of cinobufagin on the expression of CD44s in ovarian carcinoma 3AO cells cultured in vitro.Methods Ovarian carcinoma 3AO cells were cultured in vitro;after the intervention of tinobufagin with different concentration,the expression of CD44s was measured by RT-PCR,and the cellular apoptosis was tested by flow cytometry.Results Ovarian carcinoma 3AO cells expressed CD44S mRNA of 1.3±0.1 and the cellular apoptofic rate was(2.31±0.98)%:0.25 mg/ml cinobufagin did not affect CD44s mRNA and cellular apoptofic rate(P ＞0.05):when 2.5 mg/ml cinobufagin was intervened,the expression of CD44s mRNA was 1.0±0.1 and the cellular apoptotic rate Was(28.69±4.16)%,showing significant difference comparing with the control group of ovarian carcinoma 3AO ceils(P＜0.05):the intervention of 25 mg/ml cinobufagin was similar to that of 0.25 mg/ml cinobuhgin(P＞0.05).3AO cells.
