The study of pulsed-ultrasound destructed microbubble to enhance liposome gene transfection in vitro
- VernacularTitle:脉冲超声辐照微泡增强脂质体介导基因转染的体外实验研究
- Author:
Zhaoxia WANG
;
Zhigang WANG
;
Chuanshan XU
;
Jianli REN
;
Xingsheng LI
- Publication Type:Journal Article
- Keywords:
Ultrasonography;
Microbubbles;
Liposomes;
Gene transfer techniques
- From:
Chinese Journal of Ultrasonography
2008;17(11):985-988
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate whether pulsed ultrasound-mediated microbubbles destruction could effectively enhance the efficiency of liposome delivery plasmid to hepatoma cells.Methods The cultured HepG2 cells were divided into six groups.The first group was as contrast group and the second group was given proper dose of liposome and plasmid.Pulsed-ultrasound exposed liposome was the third group.Add plasmid and microbubbles to the fourth group and exposed to pulsed-ultrasound.Add plasmid and liposome to the fifth group and exposed to pulsed-ultrasound,too.Liposome with plasmid and microbubbles were applied to the sixth group and exposed to ultrasound.After 24 hours,the EGFP expression in the hepatoma cells was detected by fluorescence microscopy and MTT.Results Green fluorescence intensity and transfection efficiency of the fifth group were higher than that of other groups,and cell viability had no significant difference.Although they were high in the sixth group,cells were partly died.Conclusions The liposome delivered EGFP expression efficiency in hepatoma cells was increased with the administration of pulsed ultrasound-mediated microbubbles destruction.In certain condition,microbubbles can enhance the efficiency of plasmid.
