Study of the prevention of carboplation-induced reproduction damage by gonadotropin-releasing hormone agonist
- VernacularTitle:促性腺激素释放激素激动剂对卡铂损伤生殖功能保护作用的实验研究
- Author:
Yanfang HE
;
Ping HAN
;
Jingyao WANG
;
Fengyan XIA
;
Fengying CAO
;
Yuemin BAI
;
Xiangyang YU
- Publication Type:Journal Article
- Keywords:
Reproduction;
Gonadotropinreleasing hormone agonist;
Carboplation;
Ovarian toxicity;
Rat
- From:
Clinical Medicine of China
2008;24(12):1280-1283
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect of gonadotropin releasing hormone agonist(GnRHa)against car-boplation-induced gonadotoxicity in rats. Methods Forty Wistar rats were divided into four groups which received carboplation, GnRHa + carboplation, GnRHa and normal saline respectively(n=10 for each group). Blood samples were collected from the abdominal aorta and the levels of blood follicle stimulation hormone (FSH) and estradiol (E<2>) were determined. Both ovaries and uterus of each rat were removed to measure the amount and the maturity of follicles. Body mass and morphological and pathological features of the rats were also observed. Results Compared with that in control group, the body mass of ovary and uterus decreased (P<0.05), and a significant reduction was observed in the number of ovarian follicles at each grade (P<0.05). The levels of E2 significantly lowered (P<0.05) and the level of FSH markedly ascended in group carboplation. Compared with that in group carboplation, the amount of primitive follicles significantly increased in group GnRHa + carboplation (P<0.05), and carboplation showed markedly protective effect on the ovarian and uterine morphological construction of rats. Conclusion Gn-Rha, appliying to preventing the rat reproduction damage in advance, has the certain protective function.
