The association analysis of plasma Hcy level and MTHFR gene polymorphism with ankylosing spondylitis
- VernacularTitle:血浆同型半胱氨酸水平亚甲基四氢叶酸还原酶基因多态性与强直性脊柱炎的相关性研究
- Author:
Jinwei CHEN
;
Ni MAO
;
Wenfeng PENG
;
Jiesheng GAO
;
Fen LI
;
Jing TIAN
;
Xi XIE
;
Jinfeng DU
- Publication Type:Journal Article
- Keywords:
Spondylitis ankylosing;
Methylenetetrahydrofolate reductase(NAPDH2);
Homocysteine;
Gene polymorphism
- From:
Chinese Journal of Rheumatology
2008;12(12):822-825
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the relationship between plasma homocysteine (Hey) level and ankylosing spondylitis (AS).To analyze the association between the NS,N10 methylenetetrahydrofolate reductase (MTFHR) gene polymorphism and AS.Methods One hundred patients with AS and 60 healthy controls were included in the study.The plasma Hey level was examined by enzyme-linked immunoadsorbent assay and MTHFR gene polymorphism was analyzed by the polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP).Results Compared with heahhy controls,the plasma Hey level in AS patients was significantly higher than that of the controls (P＜0.01).There was no significant difference in the frequen-cies of MTHFR genotype and alleles between AS and the controls (P＞0.05),But the ratio of T/T genotype mutation was different between AS and the controls (P＜0.05).The plasma Hey level of T/T genotype was significantly higher than that of C/T or C/C genotype in AS and the controls (P＜0.01).Logisticalregression analysis indicated that Hey was an independent risk factor for AS (P＜0.01,0R=4.582,95%CI=1.984～10.585).Conclusion The plasma homocysteine level is significantly increased in AS patients.Hyperhomo-cysteinemia is an independent risk factor for AS.MTHFR T/T genotype mutation is an important mechanism of hyperhomocysteinemia and may be related with AS.
