Study on DNA damage and cell apoptosis in the blood vessel endothelial cell of diabetic rats
- VernacularTitle:糖尿病大鼠血管内皮细胞DNA损伤与凋亡的研究
- Author:
Yun QI
- Publication Type:Journal Article
- Keywords:
Diabetes mellitus;
Endothelium,vascular;
DNA damage;
Apoptosis
- From:
Journal of Chinese Physician
2008;10(11):1441-1443
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the relationship of DNA damage and cell apoptosis in the blood vessel endothelial cell of diabetic rats. Methods Streptozotocin was used to make diabetic rats, 16 rats were random divided into diabetic group and control group. DNA damage was measured by comet assay and cell apoptosis was detected by flow eytometry. Results DNA damage score was 29.5 ± 6.7 and 8.6±3.6 in diabetic rats and controls, the rate of cell apoptosis was respectively 10.9±3.5 and 4.4 ± 1.2. It had a significant difference between two groups in DNA damage and cell apoptosis(P < 0.05). The rat of cell apoptosis had strongly positive correlation with DNA dam-age scores (r = 0.82, P < 0.05). Conclusion DNA damage and cell apoptosis are found in diabetes mellitus, cell apoptosis may be asso-ciated with DNA damage. The roles of DNA damage and cell apoptosis in diabetes mellitus need further research.
