The experimental study of recombinant adenovirus-mediated human wild type p53 gene transfection into neuroblastoma cell line
- VernacularTitle:重组腺病毒介导人野生型p53基因转染神经母细胞瘤细胞系的实验研究
- Author:
Fang CHEN
;
Lei LIU
- Publication Type:Journal Article
- Keywords:
Genes,P53;
Neuroblastoma;
Transfection;
Cell proliferation;
Cell cycle;
Apoptosis
- From:
Journal of Chinese Physician
2008;10(11):1475-1477
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effect of transduetion of human wild type gene(wt-p53) mediated by recombinant adenovirus on cell growth, cell cycle and apoptosis of neuroblastoma (NB) cell line SH-SY5Y. Methods The NB cell line SH-SY5Y were transfected by recombinant adenovirus with green fluorescent protein(Ad-GFP). The suitable muhiplieity of infection (MOI) was determined after trans-fer efficiency was tested by flow cytometry (FCM). Both recombinant adenovirus(Ad) and recombinant adenovirus mediating wt-p53 gene (Ad-p53) were transfected into SH-SY5Y cells. SH-SY5Y cells without any transfection were used as blank group. The p53 protein expres-sion was validated by Western blot. Cell number was counted and growth curve was drawn. Both cell cycle and apoptesis were detected by FCM. Results The results indicated that the transfer efficiency of recombinant adenovirus to SH-SY5Y was satisfied, and 100 MOI was suitable. After transfection with Ad-p53, p53 protein was high expressed. Cell growth were inhibited, apoptosis and cell cycle arrest were in-duced in p53 gene transfeetion group. Conclusions Our results indicate that clinical application of the in vitro p53 gone modified tumor cell vaccine might be feasible and effective in clinical treatment of NB.
