Effects of GABA On cholangiocarcinoma cell line QBC939
- VernacularTitle:γ-氨基丁酸对人胆管癌细胞株QBC939增殖和侵袭的影响
- Author:
Qiang HUANG
;
Chenhai LIU
;
Cheng WANG
- Publication Type:Journal Article
- Keywords:
Bile duct neoplsms;
Neoplasm invasiveness;
Gamma-aminobatyric acid;
Telomerase
- From:
Chinese Journal of General Surgery
2008;23(12):960-962
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effect of GABA on the bionomics of cholangiocarcinoma cell QBC939. Methods The proliferation of QBC939 cells was investigated by MTT. The telomerase activity of QBC939 ceils was examined by modified PCR-ELISA assay. Transwell cell culture chamber assay in vitro was used to detect the ability of invasion of cholangiocarcinoma QBC939. The expression of matrix metalloproteinase (MMP) mRNA and enzymatic activity of QBC939 cells were detected by reverse transcription polymerase chain reaction(RT-PCR) and gelatin zymography, respectively. Results GABA at all tested concentration inhibited the growth and proliferation of QBC939 cells in a time-independent manner, Telomerase activity was inhibited by GABA [(0.82 ± 0.05) vs. (0.56 ± 0.05), (P<0.05)]. GABA inhibited tumor cells' trans-Matrigel ability (at 100 μmol/L GABA, cell's number decreased from 60 ± 10 to 43 ±4, P<0.05) ,and significantly inhibited the activity and expression of MMP(MMP-2、MMP- 9) in a dose dependent way. Conclusions GABA can inhibit the growth、invasion and metastasis of cholangiocarcinoma QBC939. The down-regulation of the activity of Telomerase and MMP may be involved inthis mechanism.
