Effects of DNMT3b on expression and methylation status of promoter region of DLC-I in human hepatocellular carcinoma cell line
- VernacularTitle:DNMT3b对肝癌细胞株中DLC-1的表达及启动子区甲基化状况的影响
- Author:
Jiaxiang WANG
;
Huairan LIU
;
Zhengjun FAN
- Publication Type:Journal Article
- Keywords:
Carcinoma,hepatocellular;
Methylation;
DNMT3b;
DLC-1
- From:
Chinese Journal of Hepatobiliary Surgery
2008;14(12):884-886
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effects of DNMT3b on the expression and methylation sta-tus of the promoter region of DLC-1 in human hepatocellular carcinoma cell line. Methods The SMMC-7721 cell line was divided into 2 groups. The cell line in the experimental group was transfect-ed with DNMT3b siRNA, while that in the control group was transfected with control siRNA. West-ern blot was used to detect the expression of DNMT3b and DLC-1 and MSP was employed to examine the methylation status of the promoter region of DLC-1. Results The expression of DNMT3b was significantly higher in the experimental group than in the control group, while the expression of DLC-1 was just opposite. There was no significant difference in the methylation status of the promoter re-gion of DLC-1 between the 2 groups and both were methylated. Conelnsion The inhibition of expression of DNMT3b by siRNA method can enhance the expression level of DLC-1, and the methylation status of the promoter region of DLC-1 does not change at the same time. When affecting the expression of DLC-1, DN-MT3b might not play the role of methyhransferase, but can act as a transcriptional regulatory factor.
