Effects of carbachol on intestinal inflammation and mucosal blood flow in rats with gut ischemia-reperfusion
- VernacularTitle:卡巴胆碱对缺血再灌流大鼠肠黏膜组织炎症反应和血流量的影响
- Author:
Xiaofang ZOU
;
Shaowei WU
;
Guoyong ZHOU
;
Sen HU
;
Yi LV
;
Junyou LI
;
Zhiyong SHEN
- Publication Type:Journal Article
- Keywords:
Carbachol/PD;
Reperfusion injury;
Inflammation;
Tumor necrosis factor-alpha;
Blood volume
- From:
Journal of Chinese Physician
2008;10(12):1598-1601
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of carbachol on intestinal inflammation and mucosal blood flow after gut ischemia-repedusion(I/R) in rat. Method A jejunal sac was formed in Wistar rats. The superior mesenteric artery (SMA) was occluded for 45 mi-nutes followed by 240 minutes of reperfusion. Animals were random divided into three groups: sham operation, L/R + saline injection (I/R + NS) and I/R + carbachol injection (0.1mg/kg, I/R + Ca). Immediately after occluded of SAM blood flow, either 0.1mg/kg of carba-chol or same account of 0.9% saline was injected into the jejunal sac. The pathological injury was observed with HE staining. The activity of DAO and content of TNF-α in intestinal mucosa tissue were determined. Mucosal blood flow was measured by laser Doppler. All measure-ments were done at 0 min, 30 min, 60 min, 120 min, and 240 min after reperfusion. Result In I/R group the activity of DAO in intestinal mucosa and mucosal blood flow deceased, meanwhile the content of TNF-α gut tissue was dramatically increased than those in sham operation (P<0.01). Severe pathological changes were observed in intestinal mucosa. After injection of carbachol, the activity of DAO and mucosal blood flow increased (P<0.01), but the content of TNF-α in intestinal mucosa were dramatically decreased (P<0.01), compared with those in I/R group. Conclusion Administration of carbachol protects intestinal ischemia-reperfusion injury by attenuating intestinal mucosa inflammation and increasing gut mueosal blood flow.
