Effect of early insulin therapy on nuclear factor-kB inflammatory pathway in liver of diabetic rat
10.3760/cma.j.issn.0578-1426.2009.01.007
- VernacularTitle:早期胰岛素治疗对糖尿病大鼠肝脏核因子-KB炎症通路的影响
- Author:
Yan BI
;
Mengyin CAI
;
Hua LIANG
;
Weiping SUN
;
Xiang CHEN
;
Yanhua ZHU
;
Xiaoying HE
;
Qiuqiong YU
;
Ming LI
;
Jianping WENG
- Publication Type:Journal Article
- Keywords:
Insulin;
Diabetes mellitus;
Liver;
NF-kappaBDOI:10.3760/cma.j.issn.0578-1426.2009.01.007
- From:
Chinese Journal of Internal Medicine
2009;48(1):17-22
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of early insulin therapy on NF-KB pathway and inflammatory cytokine responses in fiver of diabetic rat.Methods NF-KB p65 DNA binding was assayed with ELISA-based assay kit,cytokine gene expressions were quantified with real-time PCR and phosphoenolpyruvate carboxykinase(PEPCK),NF-KB and inhibitor KB(IKBα)protein levels wlere assayed with Westem blot.Results Compared with control,hepatic PEPCK protein level in the untreated diabetic rat increased by 40%.Early insulin and gliclazide treatment normalized PEPCK protein level.The abundance of IKBα protein was significantly decreased and nuclear NF-KB p65 DNA binding activity was incteased in untreated diabetic rats.IKBot protein content increased and NF-KB p65 DNA binding decreased during early intervention treatment.mRNAs encoding IL-1β and TNFα were increased,which were reduced to normal levels after insulin and gliclazide treatment.Conclusions It is suggested that early insulin treatment inhibits NF-KB activity and inflammatory cytokine responses in fiver that are involved in the aniefioration of insulin resistance in diabetic rats.Such results misht be due to indirect antiinflammatory effects of insulin thus relieving glucotoxicity and lipotoxicity in pefipherM tissues.
