Osteogenic and adipogenic differentiation of bone marrow-derived mesenchymal stem cells in patients with aplastic anemia
10.3760/cma.j.issn.0578-1426.2009.01.013
- VernacularTitle:再生障碍性贫血患者骨髓间充质干细胞成脂和成骨分化能力的变化
- Author:
Haiyan WANG
;
Tianling DING
;
Yi XIE
;
Xiaoping XU
;
Long YU
;
Tong CHEN
- Publication Type:Journal Article
- Keywords:
Anemia,aplastic;
Mesenchymal stem cell;
Adipocytes
- From:
Chinese Journal of Internal Medicine
2009;48(1):39-43
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the osteogenie and adipogenic difference of bone marrowdeftved mesenchymal stem cells(MSCs)between patients with aplastic anemia(AA)and healthy volunteers and to explore the role of MSCs adipo-differentiation in the pathogenetic mechanism of AA.Methods MSCs were isolated from bone marrow of patients with AA and healthy donors and expanded in vitro.MSCs derived from the AA patients and healthy volunteers were compared with respect to morphology,in vitro proliteration capacity,phenotype,differentiation ability and gene expression during differentiation.Results The MSCs clones in the AA patients were(19.30±4.77)/(5×105 MNCs)7 days after culture,being significantly lower than those in the healthy volunteers,which was(47.72±3.46)/(5×105 MSCs)(P＜0.05).Compared with those the healthy donors,MSCs from the AA patients had similar proliferative capacity in the first 8 passages and then decreased in the following passages.MSCs from different sources had the same Dhenotype.MSCs from the AA patients could differentiate more easily into adipocytes but less easily and slower into osteoblasts than those from the healthy volunteers.Conclusion The increased adipogenic capacity and decreased osteogenic capacity of MSCs in AA patients may contribute to the development and progress of AA.
