Transfection of DNA-PKCS antisense oligodeoxynucleotides in the radiosensitization of lung cancer cell line A549 in vitro
10.3760/cma.j.issn.1004-4221.2009.01.073
- VernacularTitle:DNA-PKCS反义寡核苷酸转染肺腺癌细胞系A549提高放射敏感性的实验研究
- Author:
Daoli NIU
;
Changbin JIANG
;
Junjie ZHEN
;
Fen HE
;
Minghui WAN
;
Haiyan QIN
- Publication Type:Journal Article
- Keywords:
DNA dependent protein kinase;
Antiserum oligodeoxynucleotide;
Cell lines,lung neoplasms;
Radiosensitivity
- From:
Chinese Journal of Radiation Oncology
2009;18(1):73-75
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effect of DNA-PKCS antisense oligodeoxynucleotides (ASODN) on the radiosensitivity of lung cancer cell line A549 through transfecting DNA-PKcs ASODN into A549 cells.Methods DNA-PKCS ASODN and unrelated ODN were transfected into A549 cells (testing group and control group).These cells were irradiated with 0,0.5,1.0,2.0,4.0,6.0 or 8.0 Gy X-ray.Clo nogenic assay was performed to determine the survival fraction.The parameters Do,Dq and N for the multi target single-hit model,as well as the parameters a,13 and SF2 for the linear-quadratic model,were calculated to evaluate cell radiesensitivity.ResultsIn the control group and testing group,the α value was 0.14 and O.31 ,The βvalue was 0.030 and 0.018 ,the SF2 was 0.63 and 0.41 ,the Do was 2.38 and 2.09 ,the Dq was 1.43 and 0.60 ,respectively.In the testing group,the α value of A549 cells was increased,but the β value, SF2,DO and Dq were decreased.Conclusions DNA-PKCS ASODN can enhance the radiosensitivity of A549 cells and is a potential target in treating lung cancer.
