The effect of c-myc antisense oligodeoxynucleotide on the proliferation and invasion of QBC939 cells
- VernacularTitle:C-myc反义寡核苷酸对人胆管癌细胞增殖和侵袭力的影响
- Author:
Yifei WU
;
Zhuori LI
;
Xianhai MAO
;
Jinshu WU
- Publication Type:Journal Article
- Keywords:
Genes,mye;
Oligonucleotide,antisease;
Bile duct neoplasms;
Cell proliferation;
Neoplasm invasiveness
- From:
Journal of Chinese Physician
2008;10(12):1602-1604
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of c-myc ASODN on the proliferation and invasion of human bile duct carcinoma cell line QBC939. Methods QBC939 cells was conventionally cultured. C-myc ASODN was designed and transfected into QBC939 cell line. MTT assay and transwell experiment were used to study cell proliferation and invasion of QBC939 cells. Results MTT assay showed that cell survival rate in ASODN group was significantly lower than that in blank group(P < 0.05). Transwell experiment showed that the num-ber of cells penetrated in ASODN group was significantly lower than that in blank group(P<0.01). The cell survival rate and the number of cells penetrated in vechicle group had no difference with blank comparison group(P>0.05). Conclusions C- myc ASODN can inhibit the proliferation and invasion of QBC939 cells.
