Study of genistein on human breast cancer cells of estrogen-like activity
- VernacularTitle:染料木黄酮对人乳腺癌细胞雌激素样作用机制研究
- Author:
Haipeng SHENG
- Publication Type:Journal Article
- Keywords:
Genistein/PD;
Breast neoplasms;
Cell proliferation;
Estrogens
- From:
Journal of Chinese Physician
2008;10(12):1631-1633
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the role of genistein (genistcin, GEN) on human breast cancer cell proliferation and its mecha-nism. Method GEN MTT method was used to detect the proliferation of GEN on estrogen-dependent breast cancer cells MCF-7 and non-es-trogen-dependent breast cancer cell proliferation of MDA-MB231. Estrogen receptor.antagonist IC1182780 was used to observe the effect of GEN. FACS was used to analyze the cell cycle of breast cancer cells. Results GEN promote MCF-7 cell proliferation, and make G1/S transition. Estrogen receptor antagonist can block the proliferation effect on MCF-7 cells but no effect on MDA MB231. Conclusion GEN has estrogen-like activity, which was mediated by estrogen receptor (ER).
