The treatment of olfactory ensheathing cells-neurotrophin-3 gene engineering cell transplantation on experimental allergic encephalomyelitis
10.3760/cma.j.issn.1006-7876.2009.01.010
- VernacularTitle:逆转录病毒介导神经营养因子-3基因转染嗅鞘细胞移植对自身免疫性脑脊髓炎的治疗作用
- Author:
Shougang GUO
;
Yifeng DU
;
Chuanqiang QU
;
Minzhong WANG
;
Zhouping TANG
;
Suming ZHANG
- Publication Type:Journal Article
- Keywords:
Encephalomyelitis,autoimmune,experimental;
Gene therapy;
Neurotrophic 3;
Gene transfer techniques
- From:
Chinese Journal of Neurology
2009;42(1):34-37
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the repair mechanism of olfactory ensheathing cells (OECs)-neurotrophin-3 (NT-3) gene engineering cell on neuron myeline and axon of experimental allergic encephalomyelitis (EAE).Methods OECs-NT-3 gene engineering cell, constructed by ueurotrophin-3 transinfecting OECs inducted by retrovirus, was transplanted into lateral ventricle.The migration and distribution were observed and compared with control group and OECs transplantation group.Then myeline repair and axon regeneration were evaluated in the aspects of function score, morphological structure, SYN grey level Results (1) OECs-NT-3 could survive, diffuse, migrate with axons, spread in the focus diffusely on the 28th day after transplantation.(2) OECs-NT-3 survived and migrated to the transcription level of NT-3 mRNA in transgene group, being (212.3±16.1)×10-2, significantly higher than OECs group ((1.98±0.19)×10-2) and the contrast group ((1.23±0.13)×10-2, t = - 31.161, -31.928, P < 0.01).(3) The myeline of transgene group was kept complete and the number of inflamatory focus was lower than those of other groups (t = 11.388-22.728, P <0.01).(4) The SYN grey level of transgene group was obviously higher (P < 0.01).Conclusion OECs-NT-3 cell expresses NT-3 in EAE stably and effectively, which contributes to the repair of myeline and the regeneration of axon.
