Effects of the serum of patients with congestive heart failure on the apoptosis of endothelial progentior cells and protective role of carvedilol
10.3760/cma.j.issn.1671-0282.2009.01.018
- VernacularTitle:充血性心力衰竭患者血清对内皮祖细胞凋亡的影响及卡维地洛对凋亡的干预作用
- Author:
Binggong LI
;
Menghong WANG
;
Zeqi ZHENG
;
Jingtian PENG
- Publication Type:Journal Article
- Keywords:
Congestive heart failure;
Serum;
Endothelial progentior cells;
Carvedilol;
Metoprolol
- From:
Chinese Journal of Emergency Medicine
2009;18(1):65-68
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of the Senlm of patients with congestive heart failure(CHF) on the apoptosis of endothelial progentior eelts(EPCs)and protective role of carvedil01.Method Totally 20 patientswithCHF、^mre enrolledfromDepartment ofCardiology ofthe FirstHospital Affdiated ofNanchangUniversity between June and August 2007.Serum WflflL isolatedfrom blood ofthe patients.1lononuelear cells were isolated from blood of20 healthy volunteers by density gradient centrifugation.Attaching cells wel-e harvested after culture for 7 relays and the EPCs were anyzea by flow cytometry and immunofluorescenee method.EPCs were divided into 4 groups:group A,cultured in sE-of volunteers;group B,cultured in serum of CHF;group C,cultured in leNlm of CFIF and Carvedilol;group D,cultured in,erum of CI-IF and metoproM,After euhure for 24 hours,annex.inV/PI wss used to investigate the apoptosis rate ofEPCs and the activity ofcllspafle 3 were exanlilled.ELISA Wills used to detect the level of flerum TNF-a of patients with CHF.Multiple group compalrion Wills conducted with variance analvsis and q test.Results The apoptosis rate and easpase 3 activity of EPCs in group B[(7.5 4-0.9)%v8.(3.0 4-0.25)]were significantly ineased compared with group A[(3.4±0.7)%VS.(1.37±0.2),P
