Endothelial progenitor cells pretreated ex vivo with SDF-1 improve their therapeutic efficiency for acute myocardial infarction
10.3760/cma.j.issn.1671-0282.2009.02.013
- VernacularTitle:基质细胞衍生因子预处理的内皮祖细胞对大鼠急性心肌梗死的治疗作用
- Author:
Lang WANG
;
Hong JIANG
;
Sisi CHEN
;
Yuanyuan ZHANG
;
Lihua ZHU
- Publication Type:Journal Article
- Keywords:
Stromal cell-derived factor-1;
Endothelial progenitor cells;
Myocardial infarction;
Pretreat merit
- From:
Chinese Journal of Emergency Medicine
2009;18(2):161-164
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the therapeutic efficiency of intravenous implanted endothelial progeni-tor cells (EPCs) pretreated with stromal cell derived factor-1 (SDF-1) in rat model of acute myocardial infarction (AMI). Method The bone-marrow derived EPCs were cultured. The effects of SDF-1 on the migration and sur-rival of EPCs were evaluated in vitro. The rat models of AMI were produced and randomly divided into SDF-1 +EPCs group (n=12), EPCs group (n=12) and control group (n=12). EPCs pretreated with or without SDF-1 were infused via tail vein 24 hours after AMI modelled. The EBM-2 culture medium without cell was infuaed in control group. Vessel density, cardiac function and infarct area were measured on 14 days and 28 days after cell implantation. Results Fourteen days after cell implantation, the vessel density of SDF-1+EPCs group was higher than that of EPCs group and control group. Twenty-eight days after cell implantation, the cardiac function of SDF-1+EPCs group was better than that of EPCs group and control group. Conclusions EPCs pretreatment with SDF-1 can improve the survival and migratory capacity of EPCs, and increase the therapeutic efficiency for myocardial infarction.
