Effect of double activation on the development of frozen-thawed oocytes after intracytoplasmic sperm injection
10.3760/cma.j.issn.0529-567x.2009.02.014
- VernacularTitle:兔卵母细胞胞质内单精子注射后二次辅助激活对兔卵母细胞发育能力的影响
- Author:
Jing WANG
;
Lin CONG
;
Zhiguo ZHANG
;
Yunxia CAO
;
Zhaolian WEI
;
Ping ZHOU
;
Jihua ZHAO
;
Xiaojin HE
- Publication Type:Journal Article
- Keywords:
Cryopreservation;
Oocytes;
Sperm injections,intracytoplasmic;
Reproductive techniques,assisted;
Embryonic development
- From:
Chinese Journal of Obstetrics and Gynecology
2009;44(2):135-138
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the influence on developmental potential of frozen-thawed rabbit oocytes with double assisted activation followed by intracytoplasmic sperm injection (ICSI). Methods A total of rabbit oocytes were collected and thawed after vitrification cryopreservation. Among all oocytes were cultured for 1 hour followed by ICSI. 156 Survived oocytes were divided into 5 groups randomly. I0634 single activation: 30 oocytes were added with calcium ionomycin ( I0634 ) at 5 μmol/L for 5 minutes;SrCl2single activation: 26 oocytes were added with strontium chloride at 10 mmol/L for 10 minutes;10634 double activation: 33 oocytes were activated by I0634 twice;SrCl2 double activation: 28 oocytes were activated by strontium chloride twice. Control group: 39 oocytes were not added with any activators. The rate of fertilization, cleavage and blastocysts formation were observed and compared between various groups. Result The rates of fertilization, cleavage and blastocysts formation were in group of SrCl2 single activation were higher than those of I0634 single activation group without statistical difference (54% vs. 33%, 27% vs. 17%, 8% vs. 3%, P <0.05 ). However, those above rates in double activation by I0634 were higher significantly than those of single I0634 activation (82% vs. 33%, 55% vs. 17%, 15% vs. 3%, P < 0.05). The rates of fertilization (61%) was higher and the rate of cleavage (21%) and blastocysts formation (7%) were lower in group of SrCl2 double activation in comparison with group of SrCl2 single activation without reaching statistical difference (P<0.05 ). Notably, the rates of fertilization, cleavage and blastocysts formation in I0634 double activation group were higher than those in group of SrCl2 double activation with statistical difference (82% vs. 61%, 55% vs. 21%, 15% vs. 7%, P<0.05). Conclusion It might enhance the potential of fertilization of oocytes and early embryo development treated by double activation following ICSI, however, those activated oocytes demonstrate rapid cleavage.
