Application of surface enhanced laser desorption-ionization protein chip in judging injury severity of severe traumatic brain injury

VernacularTitle:表面增强激光解析离子化蛋白芯片技术在重型颅脑创伤伤情判断中的应用
Author: Sai ZHANG ; Xiaozhi LIU ; Zhenlin LIU ; Tiezhu MA ; Lei CHEN ; Shizhong SUN
Publication Type:Journal Article
Keywords: Brain injuries; Proteemics; Protein chip
From: Chinese Journal of Trauma 2009;25(1):29-31
CountryChina
Language:Chinese
Abstract: Objective To judge injury severity of severe traumatic brain injury (sTBI) by using surface enhanced laser desorption-ionization (SELDI) protein chip technique. Methods Serum sam-ples from sTBI patients were used to detect expression of differential proteins by protein chip CM10 and SELDI to analyze the correlation between expression peak intensity and GCS. Results We obtained 101 protein peaks, with statistical difference upon expression of 27 protein peaks, when negative correla-tion was found between two peaks ( m/z 4 972 and m/z 5 322 ) and GCS score and positive correlation be-tween six peaks (m/z 3 941, m/z 4 295, m/z 8 714, m/z 8 792, m/z 14 020 and m/z 28 148) and GCS score. Conclusion SELDI protein chip technique may become a new and objective detection method in judging injury severity of sTBI.