Molecular mechanism of SH2-Bβ in regulating JAK2/STAT3 during development of obesity

VernacularTitle:SH2-Bβ调控JAK2/STAT3在肥胖症发病中的分子机制
Author: Chaojun DUAN ; Cui LI ; Cane TANG ; Jing WU ; Faqing TANG ; Zhuchu CHEN ; Zhiqiang XIAO
Publication Type:Journal Article
Keywords: SH2-Bβ; Obesity; Leptin signal pathway
From: Chinese Journal of Endocrinology and Metabolism 2009;25(1):90-91
CountryChina
Language:Chinese
Abstract: The molecular techniques were used to analyse tyrosine phosphorylation of JAK2 and STAT3 in leptin receptor overpression cell lines and SH2-Bβ knockout (SH2-Bβ-/-) mice. The serum level of leptin in SH2-Bβ mice was measured by ELISA. The results showed that SH2-Bβ dramatically enhanced the leptin-stimulated tyrosine phosphorylation of JAK2 and STAT3 in vitro. Leptin-stimulated activation of JAK2 and phosphorylation of STAT3 were significantly impaired in hypothalamus of SH2-Bβ-/- mice. The fasting and postprandial serum levels of leptin and body weight were markedly increased in SH2-Bβ-/- mice. Therefore, SH2-Bβ is an endogenous enhancer of leptin sensitivity and regulates body weight via leptin/ JAK2/STAT3 pathway.