Effects of arachidonic acid on L-type calcium channel and its mechanism of antiarrhythmia
10.3760/cma.j.issn.1671-0282.2009.02.017
- VernacularTitle:花生四烯酸对L-型钙通道的作用及其抗心律失常机制
- Author:
Chengyun LIU
;
Guiqing CHEN
;
Xiaojing GENG
;
Xin CHEN
;
Jingjing WAN
- Publication Type:Journal Article
- Keywords:
Arachidonic acid;
Ventricular myocytes;
Arrhythmia;
L-type calcium channel;
Patch clamp
- From:
Chinese Journal of Emergency Medicine
2009;18(2):180-184
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the influence of arachidonic acid (AA) on L-type calcium channel in rabbits sin-gle cardiomyocyte and its mechanism of antiarrhythmia. Method The single ventricular cardiomyocyte was isolat-ed by using enzyme dispersion method and whole-cell clamp-patch technique was used to record L-type calcium current.All data were analyzed using ANOVA. Results AA inhibited Ica-L in a concentration-dependent manner. The application of 3 μmol/L, 10 μmol/L and 20 μmol/L arachidonic acid reduced the density of peak Ica-L from (10.79±0.93)pA/pF to (8.99±0.43)pA/pF to (7.60±0.35)pA/pF and to (5.60±0.30)pA/pF, respctive-ly (n=7, P<0. O1 ). The Ica-Lpartially resumed after washout. The AA up-shifted the I-V curves of Ica-L without changes of their shape,peak and reverse potentials. The AA also markedly shifted the inactivation curve to left, and prolonged the recorvery time from inactivation,but did not change the curve of calcium channel activation. Con-clustions By acceleration of L-type calcium channel inactivation and prolongation of recorvery time from inactiva-fion,arachidonic acid can reduce the calcium ion influe and prolong effective refractory period, playing the role of antiarrhythmia.
