Antibiotic resistance and staphylococcal cassette chromosome mec genotype of Staphylococcus aureus in nosocomial infection

VernacularTitle:医院感染金黄色葡萄球菌的耐药性和SCCmec基因型分析
Author: Dongmei MU ; Peng ZHANG ; Wenfang ZHANG ; Shan ZHENG ; Yunde LIU ; Shangwei WU
Publication Type:Journal Article
Keywords: Staphylococcus auFeus; Polymerase chain reaction; mecA gene; SCCmec genotype
From: Chinese Journal of Clinical Infectious Diseases 2009;2(1):35-38
CountryChina
Language:Chinese
Abstract: Objective To investigate antibiotic resistance and staphylococcal cassette chromosome mec(SCCmec)genotype of Staphylococcus aureus isolated from patients with nosocomial infections.Methods Antibiotic susceptibility pattern anti the gene mecA were examined in 89 Staphylococcus aureus isolates from patients with nosocomial infection in Tianjin Medical University Cancer Institute and Hospital.The SCCmec genotype in the mecA-positive strains were fuaher investigated with multiplex PCR.Results In 89 Staphylococcus aureus strains,21(23.6%)were phenotypic methicillin-resistant Staphylococcus aureus (MRSA)and 39(43.8%)were rnecA positive,the difierence was of statistical significance(χ2=8.146,P=0.004).MRSA strains showed multiple-antibiotic resistance but sensitive to vancomycin.Tvpe Ⅲ SCCmec carriers were the major epidemiological strains(26/39,66.7%).ConclusionMRSA strains isolated from Tianjin Medieal University Cancer Institute and Hospital are characterized by multi-drug resistance,and type Ⅲ is the dominant SCCmec earried by the MRSA strains.