Effects of endothelin-1 on the proliferation of human HCT-116 cells
10.3760/cma.j.issn.1673-9752.2009.01.022
- VernacularTitle:内皮素1对大肠癌HCT-116细胞增殖的影响
- Author:
Xuelian ZHENG
;
Yuan LI
;
Zongguang ZHOU
;
Ling WANG
;
Jun GU
;
Keling CHEN
;
Xiao JIANG
- Publication Type:Journal Article
- Keywords:
Colorectal neoplasms;
HCT-116 cells;
Endothelin-1
- From:
Chinese Journal of Digestive Surgery
2009;8(1):60-62
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of endothelin-1 on the proliferation of HCT-116 cells. Methods The expression of endothelin-1 and endothelin receptors A and B in the HCT-116 cells was detected by RT-PCR and immunohistochemistry method.The effects of endothelin-1 at different concentrations and action time points on HCT-116 cells were measured by MTT assay and were analyzed by variance analysis.Results The expression of endothelin-1 and endothelin receptors A and B at the gene and protein levels in HCT-116 cells was detected.Endothelin-1 had significant effects on the proliferation of HCT-116 cells.The proliferation peak of HCT-116 cells was reached when the concentration of endothelin-1 was 1×10-7mol/L and the action time was 48 hours.Conclusions Endothelin-1 promotes proliferation of HCT-116 cells in a dose-and time-dependent manner,and the process is mediated by endothelin receptors.
