Influence of smoking on the gene expression of insulin receptor substrate 2 in rat muscle tissue
10.3760/cma.j.issn.1000-6699.2009.01.029
- VernacularTitle:吸烟对大鼠骨骼肌胰岛素受体底物2基因表达的影响
- Author:
Jiwang WANG
;
Suhua ZHANG
;
Zhihong WANG
;
Wei REN
;
Haojie WU
;
Juan DU
- Publication Type:Journal Article
- Keywords:
Insulin resistance;
Insulin receptor substrate 2;
Muscle,skeletal;
Smoking
- From:
Chinese Journal of Endocrinology and Metabolism
2009;25(1):94-95
- CountryChina
- Language:Chinese
-
Abstract:
RT-PCR and immunohistochemistry were used to measure the mRNA and protein expression of insulin receptor substrate 2 (IRS-2) in the muscle of smoking rats. The mRNA and protein expressions of IRS-2 in the smoking subgroups fed with normal diet or high-fat diet were significantly lower than those in corrsponding control groups (0.27±0.02 vs 0.41±0.25, 0.40±0.04 vs 0.51±0.02 for mRNA; 2.91±0.42 vs 4.90±0.29, 2.43±0.36 vs 3.80±0.30 for protein, all P<0.01). There were no differences in mRNA and protein expressions of IRS-2 between the smoking group of diabetic rats and the matched control rats (P>0.05). The variation of gene expression of IRS-2 may be involved in the mechanism of insulin resistance caused by smoking.
