Effects of cyclooxygenase-2 in ischemia reperfusion injury in liver transplantation in rat
10.3760/cma.j.issn.1673-9752.2009.01.016
- VernacularTitle:环氧合酶2在大鼠肝移植缺血再灌注损伤中的作用
- Author:
Yumin LI
;
Xiaoliang ZHU
;
Xun LI
;
Wence ZHOU
;
Bin LI
;
Bin SHI
- Publication Type:Journal Article
- Keywords:
Liver transplantation;
Cyclooxygenase-2;
Ischemia reperfusion injury;
Rat
- From:
Chinese Journal of Digestive Surgery
2009;8(1):43-46
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expression and role of cyclooxygenase-2(COX-2)in the ischemia reperfusion in"ury in liver transplantation in rat.Methods Fifty-four male Wistar rats were divided into control group(n=6),liver transplantation group(n=24)and NS398 treated group(n=24)according to random number table.The expression of COX-2 mRNA was detected by RT-PCR,the distribution of COX-2 positive cells by immunohistochemistry.and the content of serum TNF-αand IL-10 by enzyme-linked immunosorbent assay.All the data were processed by one-way ANOVA.Results The serum indexes and changes of TNF-α.expression were in accordance with the histological injury of liver graft.The expression of COX-2 mRNA was essential to the ischemia reperfusion injury of the liver graft.Inhibition of the expression of COX-2 mRNA would aggravate the isehemia reperfusion injury of the liver graft.Conclusions COX-2 plays an important role in the ischemia reperfusion injury in liver transplantation.COX-2 may decrease the isehemia repertusion injury of liver graft.
