Elevated circulating free fatty acids level causes pancreatic islet β-cell dysfunction via oxidative stress
10.3760/cma.j.issn.1000-6699.2009.01.004
- VernacularTitle:高游离脂肪酸血症通过氧化应激导致胰岛β细胞功能受损
- Author:
Xuane ZHANG
;
Yerong YU
;
Liu KE
;
Xiangxun ZHANG
- Publication Type:Journal Article
- Keywords:
Fatty acids,nonesterified;
Islet β-cell function;
Oxidative stress
- From:
Chinese Journal of Endocrinology and Metabolism
2009;25(1):13-16
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the effects of elevated circulating free fatty acids (FFA) level on basal and glucose stimulated insulin secretion (GSIS) of islet β-cell and to explore the pathophysiological link between FFA and impaired β-cell dysfunction. Methods Male SD rats underwent infusions with normal saline (C group), intralipid+heparin (FFA group) and N-acetylcysteine+FFA (NAC group) for 2-4 days. Insulin secretion from pancreatic tissues was evaluated during intravenous glucose tolerance test and isolated pancreas perfasion test at the end of 2 and 4 days infusion. Results After 2 days infusion, the basal insulin secretion from isolated perfused pancreas was increased in FFA group [(55.5±19.4 vs 27.4±6.7) mU/L, P<0.01], but the response to 16.7 mmol/L glucose in isolated perfased pancreas was similar in FFA and C groups. The peak value during GSIS was inhibited by 4 days FFA infusion [(46.8±33.0 vs 214.7±27.4)mIU/L,P<0.05]. GSIS was also decreased in FFA group compared with C group in IVGTr. After interfered with NAC, GSIS was partly recovered [(165.4± 14.8)mIU/L, P<0.01]. Conclusion Elevated circulating FFA levels may contribute to the abnormality of pancreatic islet β-cell through oxidative stress.
