The expression levels of interferon inducible genes in peripheral blood cells and their correlation with clinical features in human systemic lmpus erytbematosus
10.3760/cma.j.issn.1007-7480.2009.02.004
- VernacularTitle:系统性红斑狼疮外周血干扰素积分与临床相关研究
- Author:
Xiaoqing CHEN
;
Qiong FU
;
Yueying GU
;
Shuang YE
;
Huijuan CUI
;
Yanzhi GUO
- Publication Type:Journal Article
- Keywords:
Lupus erythematosus,systemic;
Type I interferon;
Biomarker
- From:
Chinese Journal of Rheumatology
2009;13(2):84-88
- CountryChina
- Language:Chinese
-
Abstract:
Objective To correlate the expression levels of interferon inducible genes (IFIGs) with disease activity and clinical features in systemic lupus erythematosus (SLE) patiems,.Methods Peripheral blood cells obtained from 67 SLE patients and 23 healthy donors (HDs) were subjected to real-time PCR to measure the transcriptional levels of five IFIGs (OAS-1,Mx-1,Ly6e,IFIT1 and IFIT4).Interferon scores were calculated and were compared between various groups of SLE patients as well as between patients and controls;ISRE lucife:rase reporter gene activity was measured in 17 of 67 patients and correlated with interferon score.Results Interferon scores were strongly correlated with ISRIE reporter gene aetivity,which represented for the type Ⅰ interferon activity in serum.The expression.levels of IFIGs and jinterferon scores were significantly elevated in SLE patients compared with HDs (P＜0.0001).Interferon scores were correlated positively with SLEDAI-2K(P=0.0006) and negatively with C3 levels(P=0.0162).Interferon scores were also significantly elevated in SLE patients with a positive anti-Sm or anti-RNP autoantibodies.Clonclusion The interferon score may be regarded as a good indicator for serum type I interferon activity in SLE and serves as a new hiomarker for disease activity in SLE patients.
