Establishment of rat orthotopic liver transplantation model with modified two-cuff technique by a single operator
10.3760/cma.j.issn.1673-4203.2009.02.006
- VernacularTitle:改良单人操作"双袖套法"建立大鼠原位肝移植模型
- Author:
Derong HUANG
;
Zhongjun WU
;
Yu ZHU
- Publication Type:Journal Article
- Keywords:
rats;
liver transplantation;
models,animal
- From:
International Journal of Surgery
2009;36(2):86-89
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the modified of methods and techniques to establish a rat model of or thotopic liver transplantion effectively by a single operator, and improve the stability. Methods On the basis of two-cuff technique of Kamada, we improved the techniques of perfusing, shearing, triming donor liver, the suturation of the superior and inferior eaval veins, and so on. Operation was performed in 80 rats with modified techniques (experiment group) and conventional techniques (control group), respectively, compare the survival rates of 48 h, 1 week, lmonth were compared between the two groups. Results In contrast to conventional teehniques, the modified techniques reduced the average time of donor operation, recipient operation and anhepatie phase (P < 0. 05), elevated 1-week, 1-month survival rates, remarkably (P < 0. 05 ).Between the Iwo groups, the survival rate of 48 hour had no statistical significance (P > 0. 05). Conclusions The modified techniques may reduce the operation time of donor and receptor effectively raise the quality of donor liver, elevated the survival rate of receptor. II is a stable, reliable and effective method to establish the rat urthotopic liver transplantation model.
