Relationship between-160C/A polymorphism in CDH1 gene promoter and protein expression in hepatocellular carcinoma
10.3760/cma.j.issn.1007-8118.2009.02.011
- VernacularTitle:肝细胞肝癌CDH1基因启动子C/A单核苷酸多态性与蛋白表达的关系
- Author:
Xujian CHEN
;
Quanfa NI
;
Haoqiang CAO
- Publication Type:Journal Article
- Keywords:
Carcinoma,hepatocellular;
Single nucleotide polymorphism;
E-cadherin
- From:
Chinese Journal of Hepatobiliary Surgery
2009;15(2):110-112
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the relationship between-160C/A single nucleotide poly-morphism(SNP) in the CDH1 gene promoter and protein expression in hepatocellular carcinoma. Methods Samples were taken from 34 patients with hepatocellular carcinoma and -160 C/A SNP of CDH1 gene promoter was detected by blood specimens adopting direct DNA sequencing. The expres-sion of E-cadherin which encoded by CDH1 gene was determined by paraffin-embedded tissue using immunohistochemistry and the association between -160 C/A SNP and protein expression was ana-lyzed. Results The high and low expression of E-cadherin was observed in 18 cases(52.9%) and 16 cases(47.1%). In these two groups, the difference of the incidence between CC genotype and CA, AA genotype was statistically significant(P<0.05) whereas there was no marked difference between CA and AA genotype. The difference of both A and C allele frequency was significant(P<0.05). Conclusion The -160 C/A SNP of the CDH1 gene promoter may play an important role in regulating the expression of E-cadherin in hepatocellular carcinoma and the incidence of A allele is associated with down-regulation of E-cadherin expression.
