Calbindin-D28K Prevents Staurosporin-induced Bax Cleavage and Membrane Permeabilization.
- Author:
Won Seok CHOI
1
;
Young J OH
Author Information
- Publication Type:Original Article
- Keywords: calbindin-D28k; calcium; calpain; neuronal cell death
- MeSH: Apoptosis; Calbindin 1*; Calcium; Calpain; Cell Death; Cell Line; Cell Membrane; Membranes*; Necrosis; Neurons; Staurosporine
- From:Experimental Neurobiology 2014;23(2):173-177
- CountryRepublic of Korea
- Language:English
- Abstract: Calbindin-D28K has been implicated in the regulation of neuronal cell death. Previously, we demonstrated that calbindin-D28K prevents staurosporine (STS)-induced caspase activation and subsequent apoptosis in a neuronal cell line. However, the role of calbindin-D28K in STS-induced activation of calpain and necrotic cell death was not identified. Staurosporine induced the elevation of intracellular calcium after 1 hr of treatment. Overexpression of calbindin-D28K and presence of a calcium chelator, BAPTA, prevented the increase of calcium in STS-treated cells. Cleavage of Bax by calpain was prevented by the overexpressed calbindin-D28K. Permeabilization of the plasma membrane, a factor in necrosis, as well as apoptotic change of the nucleolus induced by STS, was prevented by calbindin-D28K. Thus, our study suggests that calbindin-D28K may exert its protective functions by preventing calpain activation in necrotic cell death, in addition to its effect on the caspase-apoptosis pathway.
