Apoptosis in liver injury following limbs ischemia reperfusion and the protective effects of taurine
10.3760/cma.j.issn.1008-6315.2009.02.007
- VernacularTitle:肢体缺血再灌注后肝损伤中细胞凋亡的发生及牛磺酸的保护效应
- Author:
Na ZHANG
;
Fei GENG
;
Yan LIU
;
Yinhuan WANG
;
Xia ZHAO
;
Shuyun DONG
;
Lianyuan ZHANG
- Publication Type:Journal Article
- Keywords:
Liver injury;
Apoptosis;
Taurine;
Reperfusion injury;
Rats
- From:Clinical Medicine of China
2009;25(2):130-132
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the apeptosis in liver injury following limbs ischemia-reperfusion(IR) in rats and the protective effects of taurine.Methods The model of limbs ischemia-reperfusion injury was established.30 Wistar rats were randomized into 3 groups: control group,IR group and tanrine + reperfusion group (TR group) (n = 10 for each group).The levels of malondialdehyde (MDA) and xanthineoxidas (XOD), calcium and myeloper-oxidase (MPO) in the liver tissue were measured.DNA fragmentation was observed and analyzed by agarose gel e-lectrophoresis.Apoptosis was detected by TUNEL methods.The morphologic changes were observed with HE stai-ning.Results Compared with control group,the values of MDA, XOD, MPO, calcium in liver tissue were increased significantly in IR group (P<0.01), but the values of those in TR group were lower than in IR group (P<0.01).The percentage of apeptosis cell was higher in IR group than in control group(P<0.01).Compared with IR group, the percentage of apoptosis cell was lower in TR group (P<0.01).IR group presented DNA ladder pattern, while TR group showed no specific DNA ladder pattern in agarose gel electrophoresis.Conclusion Apoptosis participates in the liver injury after limb ischemia-reperfusion.Taurine can mitigate the liver injury and apoptosis after limb is-chemia-reperfusion injury in rats.
