Killing effect of 125I-UdR on human lymphoma Raji and Daudi cell Hoes
10.3760/cma.j.issn.0254-5098.2009.01.015
- VernacularTitle:125I-脱氧尿嘧啶核苷对淋巴瘤细胞Raji和Daudi的杀伤作用
- Author:
Jianmei WAN
;
Wo FAN
;
Youjiu ZHANG
;
Ran ZHU
;
Zeyang YU
- Publication Type:Journal Article
- Keywords:
Lymphoma cells;
Raji;
Daudi;
125I-UdR;
Uptake;
Killing effect
- From:
Chinese Journal of Radiological Medicine and Protection
2009;29(1):50-53
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the killing effect and the uptake of 125I-UdR on human lymphoma Raji and Daudi cell lines. Methods The amount of 125I-UdR in the cells and cell nuclei were determined after incubation of different time in RPMI 1640 culturing medium containing different concentrations of 125I-UdR. The killing effects of 125I-UdR on Raji and Daudi cell lines were estimated through MTT assay and cell cycle was analyzed by propidium iodide (PI) staining. Results The amounts of 125I-UdR in Raji and Dandi cells and cell nuclei were much higher than that of Na125I(P < 0.05). The amounts of 125l-UdR in Raji and Daudi cells were 14414±95 and (6916± 53.69) Bq/106 cell when the concentration was 100 kBq/ml. The amounts of Na125I were 68± 3.8 and (324±32.8) Bq/106 cell. The uptake of 125I-UdR in Raji and Daudi cells and cell nuclei increased with the 125I-UdR concentration and incubated time. The cell surviving fractions of 125I-UdR groups was much lower than that of Na125I groups (P < 0.05). When the concentration was 500 kBq/ml and incubated time was 48 hours, the Raji and Dandi cell surviving fractions of125I-UdR groups were (19.78 ± 1.39)% and (43.17 ± 2.69) % ;those of Na125I groups were (79.10 ± 1.79) % and (80.36 ± 6.12) %. The surviving fractions of 125I-UdR groups reduced with the 125I-UdR concentration. Conclusions 125I-UdR can be specially ingested by Raji and Daudi cells and incorporated into DNA, then the cells will be killed. The uptake of 125I-UdR is dose and time dependent.
