Experimental study of improved methods of liver lobe resection for liver transplantation in rats
10.3760/cma.j.issn.1008-6706.2009.02.020
- VernacularTitle:改进小体积肝移植供肝大鼠模型建立方法的实验研究
- Author:
Xiaoming WANG
;
Caiming ZHOU
;
Jun LI
- Publication Type:Journal Article
- Keywords:
Hepatectomy;
Liver transplantation;
Rats
- From:
Chinese Journal of Primary Medicine and Pharmacy
2009;16(2):231-232
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore an effective and viable way to establish small-for-size liver transplantation model in rats.Methods Male Lewis rats were used as donors and recipients,the grafts were implanted according to Kamada's 2-coff method.Animals were divided into the following groups:Group I (n=28):30% size graft liver transplantation using a median lobe graft,with left lobe,right lobe,caudate lobe and triangle lobe removed in situ;Group Ⅱ (n=36) :30% size graft liver transplantation using a median lobe graft,with an improved technic of lobe resection.7-day survival rat and the technical complications including bleeding,IVC or portal vein stricture and bile leakage were compared between the groups.Results Compared to the Group Ⅰ,the incidence of IVC stricture was significantly decreased in the Group Ⅱ.Other complications showed no significant difference between the two groups.No significant difference were observed in the 7-day survival rate between the two groups(33%,9/28 vs 60%,21/36),( X2 = 0.272,P ＞ 0.05 ),though Group Ⅱ had more survivors 7 days after liver transplantation.Conclusion With improved method of lobe resection,more effective and viable 30% small-for-size rat liver transplantation model can be established.
