The distribution of mesenchymal stem cells after total-body irradiation in rats
10.3760/cma.j.issn.1004-4221.2009.02.125
- VernacularTitle:骨髓间充质干细胞在全身照射大鼠体内分布实验研究
- Author:
Shoumin BAI
;
Biling LIANG
;
Zhiwei LI
;
Fei HAN
;
Chunyan CHEN
;
Taixiang LU
- Publication Type:Journal Article
- Keywords:
Mesenchymal stem cells;
Fluorescent quantitative;
Reverse transcriptase polymer-ase chain reaction;
Irradiation;
Rat
- From:
Chinese Journal of Radiation Oncology
2009;18(2):125-129
- CountryChina
- Language:Chinese
-
Abstract:
Objective To detect the distribution of mesenchymal stem cells(MSCs) after total-body irradiation in rats. Methods MSCs were cultured and labeled with green fluorescent protein(GFP). Rats were exposed to total-body irradiation(TB1) or TBI plus total brain irradiation, and then MSCs were injected through the tail vein. The Fluorescent MSCs were observed by fluorescence microscope. The MSCs numbers in different organs were determined by quantitative RT-PCR method. Results GFP-labeled MSCs were ob-tained. After MSCs were infused to the rats,few of them were observed in the organs of nonirradiated group except for a very low number in the lungs,bone marrow(BM) and spleen. TBI of 6 Gy increased the engraft-ment of MSCs in almost all the organs, especially in early response tissues such as the small intestine and BM. TBI of 7 Gy further increased the number of MSCs. The MSCs numbers in the brain and other organs were significantly increased after 20 Gy total brain irradiation in addition to 6 Gy TBI. Conclusions Radi-ation injury can induce the aggregation of MSCs. With the increase of radiation dose and severity of radiation injury,a significant increase of MSCs in different organs were observed. Local irradiation can increase the MSCs distribution in the radiation field as well as other organs.
