Effects of Ku80 on radiosensitivity and cell cycle in human esophageal carcinoma cells

VernacularTitle:Ku80对人食管癌细胞放射敏感性及细胞周期的影响
Author: Qingshan YANG ; Yong WANG ; Feiyue FAN
Publication Type:Journal Article
Keywords: shRNA; Ku80; Radiosensifivity; Cell cycle
From: Chinese Journal of Radiological Medicine and Protection 2009;29(1):44-46
CountryChina
Language:Chinese
Abstract: Objective To study the role of KuS0 in the treatment of esophageal cancer through inhibiting the Ku80 expression by shRNA. Methods shRNA-KuS0 vector was constructed. The effectiveness and feasibility of RNA interference were confirmed by Western blot and RT-PCR methods. The cell sensitivity to ganuna-rays was studied by colony formation assay. The effects of shRNA-Ku80 on cell cycle were observed by flow cytometry analysis. Results ShRNA-Ku80 vector was constructed successfully. Inhibition of Ku80 expression by shRNA enhanced the sensitivity of esophageal cancer cells to gamma-rays, shRNA K3 or shRNA H2 showed higher percentage of cells in G2/M phase (61.8% vs 28.6% ;64.3% vs 28.6%). Conclusions Inhibitions of Ku80 expression by shRNA play a role in the treatment of esophageal cancer. Ku80 might be a new target of tumor treatment.