Analysis of complete nucleotide sequence of a genotype D hepatitis B virus strain from a Uighor patient in Xinjiang
10.3760/cma.j.issn.1000-6680.2009.02.012
- VernacularTitle:一株新疆维吾尔族D型乙型肝炎病毒的全基因克隆分析
- Author:
Xiaobo LU
;
Lin XIAO
;
Yingzi TANG
;
Lin LIU
;
Yuexin ZHANG
;
Yuming WANG
- Publication Type:Journal Article
- Keywords:
Xinjiang;
Hepatitis B virus;
Genotype;
Cloning,molecular;
Gene amplification
- From:
Chinese Journal of Infectious Diseases
2009;27(2):103-108
- CountryChina
- Language:Chinese
-
Abstract:
Objective To determine the complete nucleotide sequence of a genotype D hepatitis B virus (HBV) strain from a Uighur patient with chronic hepatitis B in Xinjiang.Methods The complete nucleotide sequence of a HBV strain obtained from a Uighur patient with chronic hepatitis B was amplified by polymerase chain reaction (PCR) and cloned for sequencing.The bioinformatics analysis was done using BioEdit and National Center for Biotechnology Information Basic Local Alignment Search Tool (NCBI-BLAST).Results The complete nucleotide sequence of Xinjiang HBV strain was 3174 bp.The nucleotide homology was 92%-98% between the Xinjiang strain and the published genotype D HBV strains and 91% compared to the published genotype D/C combination HBV strains from China (AY862865).There was a 9 bp deletion from nucleotide 1760 to nucleotide 1768 (ATTAAAGGT).This Xinjiang HBV strain belonged to ayw2 subtype and D genotype without any recombination with other genotypes.In the term of evolutionary relation,the complete nucleotide sequence of this Xinjiang HBV strain was closest to those of two Turkey HBV strains (AY796032 and AY721605) among 34 genotype D strains published in GenBank.Conclusions The complete nucleotide sequence of a genotype D HBV strain from a Uighur chronic hepatitis B patient in Xinjiang is determined.The sequence shows some unique characteristics.
