Experimental study on bystander effects K562 cells induced by ionizing radiation

VernacularTitle:电离辐射致K562细胞旁效应的买验研究
Author: Xumin TU ; Xianhua GUO ; Jianlei RUAN ; Suwen LEI ; Huimin LU ; Wenjian LI ; Jufang WANG
Publication Type:Journal Article
Keywords: 60Co γ-rays; Bystander effect; K562 cell; Apoptosis
From: Chinese Journal of Radiological Medicine and Protection 2009;29(1):20-22
CountryChina
Language:Chinese
Abstract: Objective To study the bystander effects and associated mechanisms through irradiated conditioned medium(1CM). Methods Natural kilhr(NK) cells were obtained from peripheral blood samples. ICM irradiated with different doses of 60Coγ-rays was used for culturing K562 cell strain. The degree of injury of K562cells by activated NK cells was observed, as well as the apoptosis frequency of K562 cell was investigated. Results Severe injury was induced in K562 cells cultured in ICM than the control (sham-irradiated) as shown by increased sensitivity to NK cells (P < 0.05). The apoptosis frequency of K562 cell was increased significantly compared with the control cells (P < 0.05). Conclusions The bystander effect induced by irradiation is existent. ICM can trigger the bystander effect on K562 cell strains.