A dose-effect curve of premature condensation chromosome ring in lymphocytes of human peripheral blood exposed to high dose of 60Co γ-rays in vitro

VernacularTitle:60Co γ线超大剂量照射人离体血早熟凝集染色体环的剂量-效应曲线
Author: Bo YAO ; Yufang LI ; Guangxian LIU ; Shan HUANG ; Bentong JIANG ; Huisheng AI
Publication Type:Journal Article
Keywords: High dose radiation; Premature condensation chromosome ring; Dose-effect curve
From: Chinese Journal of Radiological Medicine and Protection 2009;29(1):54-56
CountryChina
Language:Chinese
Abstract: Objective To establish a dose-effect curve of premature condensation chromosome ring (PCC-R)in lymphocytes of human peripheral blood after exposed to high doses of Y-rays. Methods Peripheral blood samples was drawn from three healthy individuals, and exposed to 60Co γ-rays with doses between 0 and 30 Gy. The frequencies of PCC-R in premature condensation chromosome (PCC) cells obtained by Okadaic acid (OA) induction were calculated, and a dose-effect curve was fitted. Results PCC index tapered with dose. Frequencies of PCC-R per cell increased until 20 Gy, and then saturation was observed. The results were fitted to a lineal model up to 20 Gy: y = - 0.020 + 0.052D ,where y was the frequencies of PCC-R per cell, D was the radiation dose (Gy). Conclusins The highest dose could be estimated is 20 Gy by the dose-effect curve established with PCC-R method. Its utility and validity will be verified in the future application of radiation accident.