Association between a genomic polymorphism within the CD14 locus and severe sepsis outcome as well as cytokines
- VernacularTitle:CD14基因多态性与严重感染患者炎症介质及预后的关系
- Author:
Changqiong XU
;
Wei WANG
;
Huaining WANG
;
Dehong LIU
;
Xiaoling FENG
- Publication Type:Journal Article
- Keywords:
Antigens,CD14;
Endotoxins;
Infection;
Tumor necrosis factor-alpha;
Interleukin-6;
Interleukin-10;
Prognosis
- From:
Journal of Chinese Physician
2009;11(1):45-47
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the relation of a lipopolysaecharide receptor CDl4C-159T gene polymorphism to severe sepsis outcome and cytokines in postoperative severe sepsis.Methods A prospective,consecutive entry study was made among 42 postoperative sevel'e sepsis admitted in neurosurgeon department,Second People～Hospital ShenZhen between Feb.2007 to Jul.2007.The genomie DNA of peripheral blood nucleated cells WaS extracted.CDl4-159C/T gene polymorphism patients was detected by restrictive fragment length polymorphism(RFLP)analysis.The relationship between genotypes and the production of eytokines TNF-α,IL-6,IL-10 and mortality rate of severe sepsis were evaluated.Results The mortality of sepsis in patients with CDl4 homozygous for the T allete(Tr)(69.2％)was significanfly higher than those with genotype CT(23.1％)and C allete(CC)(7.7％).In addition,TNF-a and IL-6 production ofTI'homozygore were markedly higher than those of the TC and CC genetypes.while IL-10 production were the lowest of the three(P<0.05).Conclusion The single base pair pelymorphism at position-159 in the CD14 gene promoter might influence the outcome of severe sepsis and may be related to production of pro-inflammatory cytokines TNF-α,IL-6,and decrease the anti-inflanunatiry factor IL-10.
