Effects of adhesion mediated by bone marrow stroma cells from leukemia patient on cell cycle of Jurkat cells
- VernacularTitle:白血病骨髓基质细胞黏附对Jurkat细胞周期及调控的影响
- Author:
Jigang WANG
;
Xinghua CHEN
;
Fan ZHOU
;
Yanqing LIU
;
Dantong WU
;
Yin BAI
;
Jinhua LIU
- Publication Type:Journal Article
- Keywords:
Leukemia;
Bone marrow cells;
Cell adhesion;
Cell cycle;
Cell cycle proteins;
Drug resistance,neoplasm
- From:
Journal of Chinese Physician
2009;11(1):50-53
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of adhesion mediated by bone nlalTow stroma celh from leukemia patient on chemotherapeutics sensitivity and cell cycle of Jurkat cells in the co-cultured model.Methods Bone mw stroma cells were isohted and cultured from leukemia patients routinely.To construct the co-cultured model.Jurkat cells were co-cultured with BMSCs the irradiated layer by 60Co,and the model was observed with scanning electron microscope.The IC50 values of Jurkat cells expesured to DNR were quantified by MTT.The cell cycles of Jurkat cells after 24h-adhesion in the co-cultured model were analyzed by Facs.The expression of cyclin A,cyclin E and p27 in Jurkat cells adhered to BMSCs for 4h.24h and 48h were detected by Western blot.Results Jurkat ceUs in the co-cultured model showed a decreased sensitivity to DNR.ICSO values for normal BMSCs,leukemic BMSCs and non-adhered control were of 1.78Ixmol/L,2.30pznol/L and 0.45p,mol/L,respectively.The percentages of Go-Gl phase for leukemic BMSCs group and non-adhered control group were of 48.74％±8.77％and 27.83％壬1.86％.Respectively.The percentages of Gz-M phase for leukemic BMSCs group and non-adhered control group were of2.01％±1.17％and 20.33％±1.84％。Respectively.Compared with eomrol group,the 24h-ad- hesion mediated by BMSCs from leukemia patients up-regulated the percentage of Go-G1 phase of Jurkat cells(P
