Expression of ED1 positive cell in glomerulosclerosis in rats
- VernacularTitle:单核巨噬细胞抗原-1阳性细胞在大鼠肾小球硬化中的表达
- Author:
Huiying ZHAO
;
Wen HUANG
;
Haichang HUANG
;
Yuehong LI
- Publication Type:Journal Article
- Keywords:
Momocytes;
Macrophages;
Antigens;
Glomemlosclerosis,focal
- From:
Journal of Chinese Physician
2009;11(1):67-70
- CountryChina
- Language:Chinese
-
Abstract:
Objective To assay the expression chinse of ED1 positive cell in focal segmental glomemlosclerosis in rats,we investigated the relationship between the infiltration of mononuclear phagocytes and the progression of glomendar sclerosis.Methods We used 12 Wistar ratswhichwere dividedintotwo groups.1est group and coutrol group.Themodel offocal segmental glomerursclerosiswas ulade by in jecting PAN 9 mg/100g body weights.The rats ofcontrol group were injected 3ml 0.9％80diuln chloride.The proteinuria,serum creatinine,fipi&and protein of the rats were examined.The rata were killed at the 20th week.All the kidneys were kept and nlade into pathologic slEun-pie.1mmunohistochemical method was applied to detect the protein expression of FIN and the EDI positive cell in renal tissue of all the rats,and the number of Edl positive cell W88 counted.The results were analyzed by SPSS.Results The proteinuria of the mts in FSGS model group was significantly increased,the serum lipid ofthem was also increased.The pathology changes of the rat renal in model group showed that a part of giomemli appeared focal segmental sclerosis or all glomerular sclerosis,and the extracelhlar matrix accumulated.In the renal of model rats,the amount of EDI positive cells was significantly higher than that in normal rats(P
