Effect of naloxone hydrochloride on neuronal cell apoptosis and cerebral edema induced by experimental brain injury
10.3760/cma.j.issn.1671-0282.2009.03.016
- VernacularTitle:盐酸纳洛酮对大鼠颅脑损伤后神经细胞凋亡及脑水肿的影响
- Author:
Xiaojie WEI
;
Zaifeng CHEN
;
Xiaojun FU
;
Xinlong XU
;
Hongsong PAN
;
Qingsong XIE
- Publication Type:Journal Article
- Keywords:
Naloxone;
Brain injury;
Hydrocephalus;
Apoptosis
- From:
Chinese Journal of Emergency Medicine
2009;18(3):281-285
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of naloxone hydrechloride on neuronal cell apoptosis and cerebral edema induced by experimental brain injury.Method Animal model of brain injury was established by free-falling method of Fecney.Totally 100 SD rats were randomly divided into sham operated group(n=20),op-erated comparison group(n=40),and naloxone hydrochloride treatment group(n=40).The naloxone hy-drochloride treatment group were subdivided into four sub-groups,and naloxone hydrochloride was injected at 30 min,6 h,24 h and 48 h after brain injury respectively,while the comparison groups were injected with physiologi-cal saline at the same time point.The rats were sacrificed 7 deys after the injury.Neuronal cell apoptosis were de-tected by terminal-deoxynucleotidyl transferase mediated nick end labeling(TUNEL),and water content of brain tis-sue was examined with dry-wet methods.Data were analyzed by using spss 10.0 saftware package statistical analysis was carried out by using variance analysis.Results Gompared to the sham operated group,neuronal cell apopto-sis and water content of brain tissue were increased significantly in operated comparison group(P＜0.05).Com-pared to the operated comparison group,neuronal cell apoptosis and water content of brain tissue were decreased significantly in every naloxone hydirochloride treatment group(P＜0.05).Every sub-groups compared in naloxone hydrochloride treatment group,neuronal cell apoptosis of early injection group(30 min and 6 h after injury)were decreased significantly than late injection group(24 h and 48 h after injury,P＜0.05),but the water content of brain tissue had no difference between the early injection group and the late injection group(P＞0.05).Conclu-sions Naloxone can carry out its protective function to injuried neurecyte through alleviating neuronal cell apopto-sis and hydrecepahalus.and the effect was better as early as it used.
