Differentiation effecs on DNA polymerase beta and X-ray repair cross-complementing protein 1 expression in Eca 109 cells
- VernacularTitle:分化诱导剂对Eca-109细胞DNA聚合酶β及X-射线修复交联-补充蛋白1表达的影响
- Author:
Shikun LI
;
Jun XU
;
Kehe CHEN
;
Qingmei REN
- Publication Type:Journal Article
- Keywords:
DNA polymerase beta;
Eca-109 cell line;
X-ray repair cross-complementing protein 1
- From:
Chinese Journal of Primary Medicine and Pharmacy
2008;15(8):1267-1268
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the change of DNA polymerase beta and XRCC1's expression during malignant celI differentiation.Methods The Eca-109 cells were divided inm 2 groups:differentiation group which cultured with 8-Br-cAMP and control group.The 2 groups cells were cultured 48h simultaneously.The immunocytochemistry was performed to detect the expression of DNA polymerase beta and XRCC1.Results Compared with control group,the expression of DNA polymerase beta and XRCC1 was decreased simultaneously(P＜0.05).Conclusion The differentiation agent can down-regulate the expression of DNA polymerase beta and XRCC1,suggesting that overexpressed DNA polymerase beta and XRCC1 maybe result in mutator phenotype.
