Study on 3-nminobenzamide enhancing the radiosensitivity of human esophageal carcinoma cell strain in vitro
10.3760/cma.j.issn.0254-5098.2009.01.014
- VernacularTitle:3-氨基苯甲酰胺对人食管癌细胞放射增敏作用的实验研究
- Author:
Xinshe XIA
;
Hong ZHE
;
Jianping MA
;
Tongqiang YE
;
Zhe DING
;
Wenjun YU
- Publication Type:Journal Article
- Keywords:
Esophageal carcinoma cell strain;
Radiosemitizer;
Poly(ADP-ribose) polymerase (PARP);
Inhibitor;
3-Aminobenzamide(3-AB)
- From:
Chinese Journal of Radiological Medicine and Protection
2009;29(1):46-50
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the ability of 3-AB to sensitize the human esophageal carcinoma cell strain (CaEs-17) to radiation in v/tro and its mechanisms. Methods CaEs-17 cells were treated with 3-AB at 0, 2.5, 7.5 mmol/L and given irradiation O, 3, 6, 9, 12 Gy. 3-AB concentration in each group was made dose-survival curve using multi-target single-hit maiths model by clonogenie assay. MTT assay was performed to observe the survival of irradiated cells.comet assay and metaphase chromosome analysis were used to measure the DNA damage degree and chromosome aberration of CaEs-17 cell after 3-AB treatment and irradiation. Results Cell survival experiments showed SER of 1.21, 1.52 for 2.5 mmol/L, 7.5 mmol/L 3-AB respectively using multi-target single-hit maths model. The survival fraction of irradiated CaEs-17 cell was decreased after 3-AB treatment. DNA damage and the chromatid breakage number of irradiated CaEs-17 cells were increased after 3-AB treatment. Conclusions 3-AB, a PARP inhibitor, can enhance the radiosensitivity of human esophageal carcinoma cell strain (CaEs-17). DNA damage repair inhibition by 3-AB might be one of the mechanisms.
